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bovine calf serum  (ATCC)


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    Structured Review

    ATCC bovine calf serum
    Bovine Calf Serum, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7874 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bovine+calf+serum/Fetal+Bovine+Serum/pmc13269842-53-23-27
    Average 99 stars, based on 7874 article reviews
    bovine calf serum - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: BDNF exerts an NRF2-dependent cytoprotective function via a receptor-independent pathway.
    Article Snippet: After sacrifice, the brain of male Sprague-Dawley rats (RRID:RGD_737903) was dissected and cut into 100 μm slices and incubated in a 6-well plate filled with synthetic cerebrospinal fluid (aCSF, 146 mM NaCl, 3 mM KCl, 1.2 mM CaCl 2 , 1 mM MgCl 2 , 1.9 mM Na 2 HPO 4 , 0.1 mM NaH 2 PO 4 , pH 7.40). .. Cell culture and cell lines Hippocampal HT-22 cells (transformed cell line derived from hippocampal cells; RRID:CVCL_0321), BDNF KO NIH3T3cells (sex of cells: male; CRISPR-U technology; RRID: CVCL_E0ZU, Catalog number #YKO-M068, Ubigene, China), NIH3T3cells (sex of cells: male; RRID:CVCL_0594; Catalog number # YC-A013, Ubigene, China), and intestinal differentiated Caco-2 cells (sex of cells: male; RRID:CVCL_0025) were cultured and maintained at 37 ◦ C in an atmosphere containing 5% CO 2 in Dulbecco’s Modified Eagle’s Medium (DMEM; 41966029; Gibco/Thermo Fisher Scientific, USA) supplemented with 5–10% fetal bovine serum (F7524; SigmaAldrich/Merck group, USA) and 1% penicillin/streptomycin (15140122; Gibco/Thermo Fisher Scientific, USA) (HT-22 and Caco-2 cells) or in ATCC-formulated Dulbecco’s Modified Eagle’s Medium (DMEM; ATCC-30-2002; ATCC, USA) with 10% bovine calf serum (ATCC-30-2030; ATCC, USA) and 1% penicillin/streptomycin (NIH 3T3 and BDNF KO NIH3T3cells). ..

    Article Title: Cancer Cell Membrane-Wrapped Nanoparticles for Photothermal Therapy and Photoacoustic Imaging of Triple-Negative Breast Cancer
    Article Snippet: .. EpH4-Ev murine noncancerous breast epithelial cells (ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; ATCC) supplemented with 10% bovine calf serum (BCS; ATCC) and 1.2 μg/mL puromycin dihydrochloride 99% (VWR). ..

    Article Title: Mechanical cues guide the formation and patterning of 3D spheroids in fibrous environments
    Article Snippet: .. Murine 3T3 fibroblasts (ATCC, catalog no. CRL-1658) were cultured in Dulbecco's modified Eagle medium (DMEM, Corning, catalog no. 10-013-CV) + 10% bovine calf serum (ATCC, catalog no. 30-2030) + 1% penicillin–streptomycin (Gibco, catalog no. 15140-122). .. Immature human cardiomyocytes (Sigma-Aldrich, catalog no. SCC109) were cultured in DMEM/F-12 MEDIA (1:1, Corning, catalog no. 10-013-CV, Gibco, catalog no. 11765054) +12.5% fetal bovine serum (Corning, 35-015-CF) + 1% penicillin–streptomycin (Gibco, catalog no. 15140-122).

    Article Title: A Macrophage/Monocyte‐Related Four‐Gene Signature for Prognostic Assessment of Uveal Melanoma: BTBD6 , C2CD4B , CCL24 , and S100A4
    Article Snippet: Melanoma cell line A375 (CRL‐1619) and human epidermal melanocytes (PCS‐200‐013) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia) and cultured as follows. .. The melanocytes were cultured in the specified growth kit (PCS‐200‐042, ATCC), and A375 cells were grown in DMEM (30‐2002, ATCC) added with 10% bovine calf serum (30‐2020, ATCC). ..

    Article Title: BDNF exerts an NRF2-dependent cytoprotective function via a receptor-independent pathway.
    Article Snippet: .. Cells were cultured on 24-well plates (92124; TPP) for 2 days and then incubated with either the vehicle (control), BDNF [7.4 or 37 nM] (for HT-22 cells and NIH3T3cells) or BDNFΔC-ter [37 nM] (for HT-22 cells) and p75 NTR -blocking antibody (anti-p75 NTR ) for HT-22 cells in DMEM (41966029; Gibco/Thermo Fisher Scientific, USA for HT-22 cells; DMEM; ATCC-30-2002; ATCC, USA for NIH3T3cells) with or without 2.5 μM NRF2 inhibitor ML385 (SML1833, Sigma-Aldrich Chimie, France) for 6 h. The cells were then incubated in Fluorobrite (A1896701; Gibco/Thermo Fisher Scientific, USA) containing 1.5% fetal bovine serum for HT-22 cells (F7524; Sigma-Aldrich/ Merck group, USA) or 2% bovine calf serum for NIH3T3cells (ATCC-30-2030; ATCC, USA) and 1% L-glutamine (25030024; Gibco/Thermo Fisher Scientific, USA). .. The medium contained, or not (control), tert-butyl hydroperoxide (18 μM for HT-22 cells, 75 μM for NIH3T3cells) (458139; Sigma-Aldrich/Merck group, USA), a p75NTR-blocking antibody (anti-p75NTR) for HT-22 cells and, either vehicle (control), BDNF [ 7.4 or 37 nM], or BDNFΔC-ter [37 nM] for HT-22 cells.

    Transformation Assay:

    Article Title: BDNF exerts an NRF2-dependent cytoprotective function via a receptor-independent pathway.
    Article Snippet: After sacrifice, the brain of male Sprague-Dawley rats (RRID:RGD_737903) was dissected and cut into 100 μm slices and incubated in a 6-well plate filled with synthetic cerebrospinal fluid (aCSF, 146 mM NaCl, 3 mM KCl, 1.2 mM CaCl 2 , 1 mM MgCl 2 , 1.9 mM Na 2 HPO 4 , 0.1 mM NaH 2 PO 4 , pH 7.40). .. Cell culture and cell lines Hippocampal HT-22 cells (transformed cell line derived from hippocampal cells; RRID:CVCL_0321), BDNF KO NIH3T3cells (sex of cells: male; CRISPR-U technology; RRID: CVCL_E0ZU, Catalog number #YKO-M068, Ubigene, China), NIH3T3cells (sex of cells: male; RRID:CVCL_0594; Catalog number # YC-A013, Ubigene, China), and intestinal differentiated Caco-2 cells (sex of cells: male; RRID:CVCL_0025) were cultured and maintained at 37 ◦ C in an atmosphere containing 5% CO 2 in Dulbecco’s Modified Eagle’s Medium (DMEM; 41966029; Gibco/Thermo Fisher Scientific, USA) supplemented with 5–10% fetal bovine serum (F7524; SigmaAldrich/Merck group, USA) and 1% penicillin/streptomycin (15140122; Gibco/Thermo Fisher Scientific, USA) (HT-22 and Caco-2 cells) or in ATCC-formulated Dulbecco’s Modified Eagle’s Medium (DMEM; ATCC-30-2002; ATCC, USA) with 10% bovine calf serum (ATCC-30-2030; ATCC, USA) and 1% penicillin/streptomycin (NIH 3T3 and BDNF KO NIH3T3cells). ..

    Derivative Assay:

    Article Title: BDNF exerts an NRF2-dependent cytoprotective function via a receptor-independent pathway.
    Article Snippet: After sacrifice, the brain of male Sprague-Dawley rats (RRID:RGD_737903) was dissected and cut into 100 μm slices and incubated in a 6-well plate filled with synthetic cerebrospinal fluid (aCSF, 146 mM NaCl, 3 mM KCl, 1.2 mM CaCl 2 , 1 mM MgCl 2 , 1.9 mM Na 2 HPO 4 , 0.1 mM NaH 2 PO 4 , pH 7.40). .. Cell culture and cell lines Hippocampal HT-22 cells (transformed cell line derived from hippocampal cells; RRID:CVCL_0321), BDNF KO NIH3T3cells (sex of cells: male; CRISPR-U technology; RRID: CVCL_E0ZU, Catalog number #YKO-M068, Ubigene, China), NIH3T3cells (sex of cells: male; RRID:CVCL_0594; Catalog number # YC-A013, Ubigene, China), and intestinal differentiated Caco-2 cells (sex of cells: male; RRID:CVCL_0025) were cultured and maintained at 37 ◦ C in an atmosphere containing 5% CO 2 in Dulbecco’s Modified Eagle’s Medium (DMEM; 41966029; Gibco/Thermo Fisher Scientific, USA) supplemented with 5–10% fetal bovine serum (F7524; SigmaAldrich/Merck group, USA) and 1% penicillin/streptomycin (15140122; Gibco/Thermo Fisher Scientific, USA) (HT-22 and Caco-2 cells) or in ATCC-formulated Dulbecco’s Modified Eagle’s Medium (DMEM; ATCC-30-2002; ATCC, USA) with 10% bovine calf serum (ATCC-30-2030; ATCC, USA) and 1% penicillin/streptomycin (NIH 3T3 and BDNF KO NIH3T3cells). ..

    CRISPR:

    Article Title: BDNF exerts an NRF2-dependent cytoprotective function via a receptor-independent pathway.
    Article Snippet: After sacrifice, the brain of male Sprague-Dawley rats (RRID:RGD_737903) was dissected and cut into 100 μm slices and incubated in a 6-well plate filled with synthetic cerebrospinal fluid (aCSF, 146 mM NaCl, 3 mM KCl, 1.2 mM CaCl 2 , 1 mM MgCl 2 , 1.9 mM Na 2 HPO 4 , 0.1 mM NaH 2 PO 4 , pH 7.40). .. Cell culture and cell lines Hippocampal HT-22 cells (transformed cell line derived from hippocampal cells; RRID:CVCL_0321), BDNF KO NIH3T3cells (sex of cells: male; CRISPR-U technology; RRID: CVCL_E0ZU, Catalog number #YKO-M068, Ubigene, China), NIH3T3cells (sex of cells: male; RRID:CVCL_0594; Catalog number # YC-A013, Ubigene, China), and intestinal differentiated Caco-2 cells (sex of cells: male; RRID:CVCL_0025) were cultured and maintained at 37 ◦ C in an atmosphere containing 5% CO 2 in Dulbecco’s Modified Eagle’s Medium (DMEM; 41966029; Gibco/Thermo Fisher Scientific, USA) supplemented with 5–10% fetal bovine serum (F7524; SigmaAldrich/Merck group, USA) and 1% penicillin/streptomycin (15140122; Gibco/Thermo Fisher Scientific, USA) (HT-22 and Caco-2 cells) or in ATCC-formulated Dulbecco’s Modified Eagle’s Medium (DMEM; ATCC-30-2002; ATCC, USA) with 10% bovine calf serum (ATCC-30-2030; ATCC, USA) and 1% penicillin/streptomycin (NIH 3T3 and BDNF KO NIH3T3cells). ..

    Modification:

    Article Title: BDNF exerts an NRF2-dependent cytoprotective function via a receptor-independent pathway.
    Article Snippet: After sacrifice, the brain of male Sprague-Dawley rats (RRID:RGD_737903) was dissected and cut into 100 μm slices and incubated in a 6-well plate filled with synthetic cerebrospinal fluid (aCSF, 146 mM NaCl, 3 mM KCl, 1.2 mM CaCl 2 , 1 mM MgCl 2 , 1.9 mM Na 2 HPO 4 , 0.1 mM NaH 2 PO 4 , pH 7.40). .. Cell culture and cell lines Hippocampal HT-22 cells (transformed cell line derived from hippocampal cells; RRID:CVCL_0321), BDNF KO NIH3T3cells (sex of cells: male; CRISPR-U technology; RRID: CVCL_E0ZU, Catalog number #YKO-M068, Ubigene, China), NIH3T3cells (sex of cells: male; RRID:CVCL_0594; Catalog number # YC-A013, Ubigene, China), and intestinal differentiated Caco-2 cells (sex of cells: male; RRID:CVCL_0025) were cultured and maintained at 37 ◦ C in an atmosphere containing 5% CO 2 in Dulbecco’s Modified Eagle’s Medium (DMEM; 41966029; Gibco/Thermo Fisher Scientific, USA) supplemented with 5–10% fetal bovine serum (F7524; SigmaAldrich/Merck group, USA) and 1% penicillin/streptomycin (15140122; Gibco/Thermo Fisher Scientific, USA) (HT-22 and Caco-2 cells) or in ATCC-formulated Dulbecco’s Modified Eagle’s Medium (DMEM; ATCC-30-2002; ATCC, USA) with 10% bovine calf serum (ATCC-30-2030; ATCC, USA) and 1% penicillin/streptomycin (NIH 3T3 and BDNF KO NIH3T3cells). ..

    Article Title: Phenanthrene and its halogenated derivative inhibit adipogenesis and decrease peroxisome proliferator-activated receptor gamma (PPARγ) expression independently of aryl hydrocarbon receptor (AhR) activation.
    Article Snippet: Polycyclic aromatic hydrocarbons (PAHs) are natural by-products of incomplete combustion of fossil fuels, wood, incinerator waste, and are also used in man-made dyes, plastics, and pesticides.. Humans are mostly exposed to PAHs through air (ex. smoke inhalation), drinking water, and foods.. Phenanthrene (Phe) is the most abundant PAH found in the environment, however there are very few studies that have examined either its systemic health effects or its effects on metabolism and adipogenesis.

    Article Title: Cancer Cell Membrane-Wrapped Nanoparticles for Photothermal Therapy and Photoacoustic Imaging of Triple-Negative Breast Cancer
    Article Snippet: .. EpH4-Ev murine noncancerous breast epithelial cells (ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; ATCC) supplemented with 10% bovine calf serum (BCS; ATCC) and 1.2 μg/mL puromycin dihydrochloride 99% (VWR). ..

    Article Title: Mechanical cues guide the formation and patterning of 3D spheroids in fibrous environments
    Article Snippet: .. Murine 3T3 fibroblasts (ATCC, catalog no. CRL-1658) were cultured in Dulbecco's modified Eagle medium (DMEM, Corning, catalog no. 10-013-CV) + 10% bovine calf serum (ATCC, catalog no. 30-2030) + 1% penicillin–streptomycin (Gibco, catalog no. 15140-122). .. Immature human cardiomyocytes (Sigma-Aldrich, catalog no. SCC109) were cultured in DMEM/F-12 MEDIA (1:1, Corning, catalog no. 10-013-CV, Gibco, catalog no. 11765054) +12.5% fetal bovine serum (Corning, 35-015-CF) + 1% penicillin–streptomycin (Gibco, catalog no. 15140-122).

    Article Title: Chitosan Oligosaccharides Suppress Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes via Multi-Pathway Transcriptomic Reprogramming.
    Article Snippet: 3T3-L1 preadipocyte cells (ATCC CL-173), derived from mouse embryo, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. Cells were maintained in a preadipocyte growth medium (GM; Dulbecco’s Modified Eagle’s Medium with high glucose, DMEM-HG; Cytiva, Marlborough, MA, USA) supplemented with 10% bovine calf serum (CS; ATCC, Manassas, VA, USA), 100 units/mL penicillin, and 100 μg/mL streptomycin (P/S; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) at 37 ◦C in a humidified atmosphere of 5% CO2/95% air. .. The medium was routinely replaced every 2–3 days, and cells were subcultured using 0.25% Trypsin-EDTA solution (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) before reaching confluence.

    Incubation:

    Article Title: BDNF exerts an NRF2-dependent cytoprotective function via a receptor-independent pathway.
    Article Snippet: .. Cells were cultured on 24-well plates (92124; TPP) for 2 days and then incubated with either the vehicle (control), BDNF [7.4 or 37 nM] (for HT-22 cells and NIH3T3cells) or BDNFΔC-ter [37 nM] (for HT-22 cells) and p75 NTR -blocking antibody (anti-p75 NTR ) for HT-22 cells in DMEM (41966029; Gibco/Thermo Fisher Scientific, USA for HT-22 cells; DMEM; ATCC-30-2002; ATCC, USA for NIH3T3cells) with or without 2.5 μM NRF2 inhibitor ML385 (SML1833, Sigma-Aldrich Chimie, France) for 6 h. The cells were then incubated in Fluorobrite (A1896701; Gibco/Thermo Fisher Scientific, USA) containing 1.5% fetal bovine serum for HT-22 cells (F7524; Sigma-Aldrich/ Merck group, USA) or 2% bovine calf serum for NIH3T3cells (ATCC-30-2030; ATCC, USA) and 1% L-glutamine (25030024; Gibco/Thermo Fisher Scientific, USA). .. The medium contained, or not (control), tert-butyl hydroperoxide (18 μM for HT-22 cells, 75 μM for NIH3T3cells) (458139; Sigma-Aldrich/Merck group, USA), a p75NTR-blocking antibody (anti-p75NTR) for HT-22 cells and, either vehicle (control), BDNF [ 7.4 or 37 nM], or BDNFΔC-ter [37 nM] for HT-22 cells.

    Control:

    Article Title: BDNF exerts an NRF2-dependent cytoprotective function via a receptor-independent pathway.
    Article Snippet: .. Cells were cultured on 24-well plates (92124; TPP) for 2 days and then incubated with either the vehicle (control), BDNF [7.4 or 37 nM] (for HT-22 cells and NIH3T3cells) or BDNFΔC-ter [37 nM] (for HT-22 cells) and p75 NTR -blocking antibody (anti-p75 NTR ) for HT-22 cells in DMEM (41966029; Gibco/Thermo Fisher Scientific, USA for HT-22 cells; DMEM; ATCC-30-2002; ATCC, USA for NIH3T3cells) with or without 2.5 μM NRF2 inhibitor ML385 (SML1833, Sigma-Aldrich Chimie, France) for 6 h. The cells were then incubated in Fluorobrite (A1896701; Gibco/Thermo Fisher Scientific, USA) containing 1.5% fetal bovine serum for HT-22 cells (F7524; Sigma-Aldrich/ Merck group, USA) or 2% bovine calf serum for NIH3T3cells (ATCC-30-2030; ATCC, USA) and 1% L-glutamine (25030024; Gibco/Thermo Fisher Scientific, USA). .. The medium contained, or not (control), tert-butyl hydroperoxide (18 μM for HT-22 cells, 75 μM for NIH3T3cells) (458139; Sigma-Aldrich/Merck group, USA), a p75NTR-blocking antibody (anti-p75NTR) for HT-22 cells and, either vehicle (control), BDNF [ 7.4 or 37 nM], or BDNFΔC-ter [37 nM] for HT-22 cells.

    Blocking Assay:

    Article Title: BDNF exerts an NRF2-dependent cytoprotective function via a receptor-independent pathway.
    Article Snippet: .. Cells were cultured on 24-well plates (92124; TPP) for 2 days and then incubated with either the vehicle (control), BDNF [7.4 or 37 nM] (for HT-22 cells and NIH3T3cells) or BDNFΔC-ter [37 nM] (for HT-22 cells) and p75 NTR -blocking antibody (anti-p75 NTR ) for HT-22 cells in DMEM (41966029; Gibco/Thermo Fisher Scientific, USA for HT-22 cells; DMEM; ATCC-30-2002; ATCC, USA for NIH3T3cells) with or without 2.5 μM NRF2 inhibitor ML385 (SML1833, Sigma-Aldrich Chimie, France) for 6 h. The cells were then incubated in Fluorobrite (A1896701; Gibco/Thermo Fisher Scientific, USA) containing 1.5% fetal bovine serum for HT-22 cells (F7524; Sigma-Aldrich/ Merck group, USA) or 2% bovine calf serum for NIH3T3cells (ATCC-30-2030; ATCC, USA) and 1% L-glutamine (25030024; Gibco/Thermo Fisher Scientific, USA). .. The medium contained, or not (control), tert-butyl hydroperoxide (18 μM for HT-22 cells, 75 μM for NIH3T3cells) (458139; Sigma-Aldrich/Merck group, USA), a p75NTR-blocking antibody (anti-p75NTR) for HT-22 cells and, either vehicle (control), BDNF [ 7.4 or 37 nM], or BDNFΔC-ter [37 nM] for HT-22 cells.



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